3.3.6 Kromatografi Lapis Tipis Ana

3.3.6 Kromatografi Lapis Tipis
Analisis kualitatif dengan Kromatografi Lapis Tipis (KLT) menggunakan plat KLT Silika Gel 60 F254. Sampel ditotolkan pada pelat KLT menggunakan pipet kapiler dan dielusi dalam suatu bejana berisikan larutan eluen yang bervariasi untuk melihat bercak noda terbaik. Penotolan dilakukan pada jarak 0,5 cm dari garis batas bawah dengan jarak antar totolan ± 0,7 cm. Setelah eluen sampai pada garis batas atas (0,5 cm dari batas atas) dengan jarak eluen 4 cm maka elusi dihentikan dan plat diangkat dari chamber lalu dikeringkan. Senyawa metabolit sekunder dilihat menggunakan Lampu UV pada panjang gelombang 254 nm. Plat KLT dilihat pula dengan menggunakan pereaksi penampak noda cerium sulfat dan vanilin untuk melihat noda yang tidak terlihat pada lampu UV.

Results (English) 1:

Thin Layer Chromatography 3.3.6 Qualitative analysis by thin-Layer Chromatography (TLC) using TLC Silica Gel 60 F254. Ditotolkan sample on TLC plates using a capillary pipette and dielusi in a vessel containing a varied eluen solution to notice patches of stain best. Penotolan done at a distance of 0,5 cm from the lower boundary line with spacing totolan ± 0.7 cm. After eluen came to the line of the upper limit (0.5 cm from the upper limit) with a distance of 4 cm eluen elusi then stopped and the plate is lifted from the chamber and then dried. Compounds are secondary metabolites were seen using UV Light at a wavelength of 254 nm. TLC is seen also by using cerium sulphate penampak stain reagents and vanilin to see stains that are not visible in the UV lamp.

Results (English) 2:

3.3.6 Thin Layer Chromatography
Qualitative Analysis by Thin Layer Chromatography (TLC) using silica gel TLC plates 60 F254. Samples were spotted on a TLC plate and eluted using a capillary pipette into a vessel containing a solution of the eluent were varied to see the best staining. Penotolan done at a distance of 0.5 cm from the bottom boundary line with the distance between ± 0.7 cm Totolan. After the eluent until the upper boundary line (0.5 cm from the upper limit) with a distance of 4 cm eluent elution was stopped and the plates were then removed from the chamber and then dried. Secondary metabolites viewed using UV light at a wavelength of 254 nm. TLC plate seen also by using cerium sulfate reagent stains penampak and vanillin to see the stains that are not visible in UV light.