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1) Periodic RejuvenationRejuvenation by means of moving or updating old breeds of microbes from the culture medium to grow new ones on a regular basis, such as a month or two months. This technique is how most traditional researchers used to maintain a collection of isolates Microbe in the laboratory. This way is also used for storage and maintenance of microbial isolates unknown way long-term storage. Periodic rejuvenation is not recommended for long-term storage. This technique has a variety of constraints, including the possibility of a genetic change occurring through the selection variant, the chance of the occurrence of contaminants, and labeling failure. The constraints gives a greater chance of occurrence of loss of isolates compared with other techniques. However, many bacteria and fungi which can survive in a tube so that it tilts a sealed up to ten years or more, either at room temperature or in the refrigerator.
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