Uji Viabilitas dengan Metode Manik-

Uji Viabilitas dengan Metode Manik-manik
Langkah awal yang dilakukan pada uji viabilitas dengan metode manik-manik yaitu, sebanyk 9 ml larutan steril dimasukkan ke dalam awetan kultur yang telah berumur 11 hari. Kemudian kocok menggunakan vortex selama 10 menit lalu diambil sebanyak 1 ml dan dimasukkan ke dalam MRSB. Setelah itu, inkubasi pada suhu 37˚C selama 24 jam., jika proses inkubasi telah selesai maka akan terbentuk kultur yang siap digunakan untuk bekerja (kultur kerja). Selanjutnya, dilakukan proses pengenceran dan pemupukan kultur. Dalam proses pengenceran, sebanyak 1 ml kultur kerja diambil dan dimasukkan ke dalam tabung reaksi berisi 9 ml larutan pengencer steril (terhitung sebagai pengenceran 10-2). Lalu, sebanyak 1 ml dari pengenceran 10-2 diambil dan dimasukkan ke dalam tabung reaksi berisi 9 ml larutan pengencer steril (terhitung sebagai pengenceran 10-3), hal yang sama dilakukan terus-menerus hingga pengenceran 10-8. Kemudian, proses pemupukan secara pourplate dilakukan dari 10-5 sampai 10-8. Pada proses pemupukan, untuk 10-5 dilakukan dengan mengambil sebanyak 1 ml pada tabung reaksi pengenceran 10-5, kemudian dimasukkan ke dalam cawan petri yang telah berisi MRSA. Hal yang sama juga dilakukan untuk pemupukan kultur hingga 10-8. Setelah proses pemupukan selesai, lakukan inkubasi pada suhu 37˚C selama 48 jam, lalu hitung jumlah koloni yang tumbuh.

Results (English) 1:

Viability test with beads The first step is done on viability test with the method, i.e. beads sebanyk 9 ml sterile solution is put into the preservation of culture that has been aged 11 days. Then shake using vortex for 10 minutes then taken up as much as 1 ml and put into MRSB. After that, incubation at a temperature of 36 ˚ C for 24 hours, if the process of incubation have been completed then it will form a ready to use culture to work (work culture). Next, do the process of dilution and fertilizing cultures. In the process of dilution, as much as 1 ml cultures of work taken and put into test tubes containing 9 ml of sterile diluent solution (calculated as a dilution of 10-2). Then, as many as 1 ml of 10-2 dilution is taken and put into test tubes containing 9 ml of sterile diluent solution (calculated as a dilution of 10-3), the same thing is done continually until dilution 10-8. Then, the process of fertilization in pourplate performed from 10-5 to 10-8. On the process of fertilization, to 10-5 performed by taking as much as 1 ml reaction tubes in the dilution of 10-5, then put in a petri dish that already contain MRSA. The same is done for the fertilisation of cultures up to 10-8. After the process of fertilization is complete, do the incubation at a temperature of 36 ˚ C for 48 hours, and then calculate the number of colonies that grow.

Results (English) 2:

Viability Test Method with beads
The first step in testing the viability of the method of bead that is, sebanyk 9 ml sterile solution put in a preserved culture that has been aged 11 days. Then shake using a vortex for 10 minutes and then take as much as 1 ml and put in MRSB. After that, incubation at 37C for 24 hours. If the incubation process has been completed it will form a culture that is ready for work (work culture). Furthermore, the process of dilution and fertilization of culture. In the process of dilution, 1 ml of culture work taken and put into a test tube containing 9 ml of sterile diluent (calculated as the dilution 10-2). Then, 1 ml of a 10-2 dilution was taken and put into a test tube containing 9 ml of sterile diluent (calculated as the dilution 10-3), the same thing is done continuously until dilution 10-8. Then, the process of fertilization pourplate carried out from 10-5 to 10-8. In the process of fertilization, to be done by taking a 10-5 1 ml test tube dilution 10-5, then put in a petri dish that already contains MRSA. The same was done for the fertilization of culture up to 10-8. After fertilization process is complete, perform incubation at 37C for 48 hours, then count the number of colonies that grow.