KLT silika gel 60 GF254 diimpregnas

KLT silika gel 60 GF254 diimpregnasi menggunakan parafin 10% (v/v) dalam dietil eter. Beberapa variasi fase gerak yang dioptimasi mengacu pada campuran etanol : air (70:30 v/v) + 5% asam asetat glasial + 1% amonia 37%. Parameter kromatografi terbaik diperoleh dari pemisahan menggunakan fase gerak campuran etanol : air (95:5 v/v) + 5% asam asetat glasial + 1% dietilamin. Fase gerak ini juga memberikan pemisahan terbaik untuk ketiga senyawa dengan Rs > 1 dan α > 1. Pelat dipindai pada panjang gelombang 335 nm untuk analisis rifampisin dan 275 nm untuk analisis isoniazid dan pirazinamida menggunakan KLTspektrofotodensitometer. Metode yang diteliti dapat digunakan sebagai acuan untuk pemisahan rifampisin, isoniazid, dan pirazinamida pada sampel obat ataupun sampel biologis.

Results (English) 1:

TLC silica gel 60 GF254 diimpregnasi paraffin using a 10% (v/v) in diethyl ether. Some variations of the optimized motion phase refers to a mixture of ethanol: water (70:30 v/v) + 5% glacial acetic acid + 1% ammonia 37%. The best chromatography parameters obtained from the separation of motion phase using a mixture of ethanol: water (95:5 v/v) + 5% glacial acetic acid + 1% dietilamin. This motion also gives the phase separation of the third best for compounds with Rs > 1 and α > 1. The license plate is scanned at a wavelength of 335 nm for analysis of rifampin and 275 nm for analysis of isoniazid and pirazinamida using KLTspektrofotodensitometer. Methods examined can be used as a reference for the separation of rifampin, isoniazid, and pirazinamida on sample drugs or biological samples.

Results (English) 2:

TLC silica gel 60 GF254 impregnated using paraffin 10% (v / v) in diethyl ether. Several variations of the mobile phase is optimized refers to a mixture of ethanol: water (70:30 v / v) + 5% glacial acetic acid + 1% ammonia 37%. Parameters obtained from the best chromatographic separation using a mobile phase mixture of ethanol: water (95: 5 v / v) + 5% glacial acetic acid + 1% diethylamine. The mobile phase also gives the best separation for all three compounds with Rs> 1 and α> 1. The plates are scanned at a wavelength of 335 nm to 275 nm analysis of rifampicin and isoniazid and pyrazinamide for analysis using KLTspektrofotodensitometer. The method studied can be used as a reference to the separation of rifampicin, isoniazid, and pyrazinamide on drug samples or biological samples.