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A total of microbes on all frozen fish samples did not reach the standard of BAM, i.e. 25-250 colonies due to sample fish tilapia were placed in freezers where temperature is below 10 ᴼ C. Many bacteria do not grow at temperatures below 10 C ᴼ and psychotrophic organisms grow very slowly. Shewanella putrefaciens is one of the bacteria that can cause fish undergo canker.
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