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BAL 24 h cultures prepared then taken 2, 5 ml and input into the Na algimat streril. Mix until blended. Pour the suspension culture, and formed a small granules granules. Diankan for 1 h. Then discard the liquid and wash with a solution of NaCl 0.85% streril. Enter into two bottles. The first bottle is kept at a temperature of 5oC during the week and check out the viability of BALnya. The second bottle store at a temperature of 15 degrees Celsius.
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