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Dieback disease in Jabon seeds (Anthocephalus cadamba) by the fungus Botryodiplodia theobromae impede the regeneration of this plant. Control efforts that many do today is by using synthetic fungicides. But its use caused problems by leaving the residue on the environment and lead to pathogen resistance. Chitosan is a natural compound that is potentially as an alternative for controlling diseases in plants. These compounds have the biological properties and high mechanical including biorenewable, biodegradable, and so it is safe to lingkingan biofungsional. This study aims to test the effectiveness of chitosan in controlling B. theobromae cause dieback on Jabon seeds and test the effect of chitosan in controlling the viscosity of dieback on Jabon seeds.
This study uses chitosan from shrimp shells were dissolved in 1.5% acetic acid and test the viscosity using a viscometer Brookfielt. Test the effectiveness of chitosan solution in controlling dieback on dilakun Jabon seeds, in-vivo. The study design used is the design of two factors in a completely randomized design (factorial RAL) by a factor of spraying time and the concentration of chitosan solution. Time spraying a solution of chitosan made one day before the inoculation and one day after the inoculation while the concentration of chitosan used are 0.0%, 0.1%, 0.3%, 0.5%, 0.8% and 1.0%.
The results show that the solution of chitosan can reduce the severity disease in jabon seeds suspected because chitosan solution has a positively charged amine groups which can bind to the cell wall material which is negatively charged, causing leakage of the cell pathogens. Different parameters were observed incidence rate of the disease, the chitosan solution does not give real effect because Botryodiplodia theobromae capable of causing symptoms of dieback on all Jabon seeds. The concentration of chitosan solution and the spraying time significantly affect disease severity (P <0.05), but the two do not interact. The most effective treatment is administration of chitosan solution with a concentration of 0.1% prior to inoculation with the severity of the disease by 25%. Chitosan solution viscosity to be responsible percent dieback disease in Jabon seeds. The higher the viscosity of the solution of chitosan increased the percent of attacks. Viscosity best in suppressing the development of disease dieback Jabon seeds is 8.80 to 7.90% percent of attacks.
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