Metode yang paling sering digunakan

Metode yang paling sering digunakan adalah metode difusi. Metode difusi ini dapat dilakukan dengan 3 cara yaitu metode cakram kertas, metode lubang/sumuran dan metode silinder. Metode lubang/sumuran yaitu membuat lubang pada agar padat yang telah diinokulasi dengan bakteri. Jumlah dan letak lubang disesuaikan dengan tujuan penelitian, kemudian lubang diinjeksikan dengan ekstrak yang akan diuji. Setelah dilakukan inkubasi, pertumbuhan bakteri diamati untuk melihat ada tidaknya daerah hambatan di sekeliling lubang (Kusmayati dan Agustini, 2007). Namun, terjadi sedikit perbedaan pada praktikum kali ini. Media yang digunakan adalah Nutrient Agar (NA), lalu bakteri diinokulasi ke dalamnya dan menunggu agar tersebut menjadi padat. Kemudian pada agar dibuat lubang sebagai tempat diinjeksikannya ekstrak rempah-rempah.

Results (English) 1:

The most frequently used method is a method of diffusion. This diffusion method can be done in 3 ways IE method, a method of paper hole disc/sumuran and methods of the cylinder. Method of hole/sumuran that is making holes in so dense that have been inoculated with the bacteria. The number and location of the hole adapted to the purpose of the research, then holes are injected with an extract will be tested. After incubation, bacterial growth is observed to see whether there are areas of barriers around the perimeter of the hole (Kusmayati and Agustini, 2007). However, there was little difference in practical work, this time. The medium used is Nutrient Agar (NA), and then the bacteria inoculated into it and wait in order to be solid. Later on so that made the hole as a place of diinjeksikannya extracts of spices.

Results (English) 2:

The method most commonly used method of diffusion. This diffusion method can be done in three ways, namely paper disk method, the method holes / sinks and methods cylinders. Methods holes / sinks that is making holes in so dense that have been inoculated with bacteria. The number and location of holes adapted to the purpose of research, then the hole is injected with extracts to be tested. After incubation, the bacterial growth was observed to see whether there is any obstacle in the area around the hole (Kusmayati and Agustini, 2007). However, there is little difference in the lab this time. The media used were Nutrient Agar (NA), then the bacteria inoculated into it and wait for it solidifies. Then in order to make a hole as a place diinjeksikannya spice extracts.