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And the difference in size in diameter of the clear zone is also influenced by the media in order to be in a petri dish. The colony grew in thicker sections usually clear zone will more narrowly, on the contrary on the part of the thin clear zone width bigger. To avoid this, it should be noted that at the time the media pouring agar into petri dish should be kept to a thick media in petri dish evenly.This experiment used 2 bacteria, i.e. Escherichia coli and Serratia marcescens.
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