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After an hour and a alginat Ca-washed with sterile physiological solution to clean the rest of CaCl2 solution is attached to the surface of the gel and the osmosis pressure also to keep microbes in question because of the concentration of a compound that was found in the bacteria isotonik.The size and a grain-grain benuk alginat depending on the viscosity of the solution and a alginat-Na penetesan distance. The use of an initial concentration of Na-alginat over 2% penetration of nutrient would reduce cell growth because gel becomes stiff.
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