3.3.5 Uji Perkecambahan Biji Kacang

3.3.5 Uji Perkecambahan Biji Kacang Hijau (Vigna radiata) dan Padi (Oriza sativa).
Ekstrak ditimbang dan dilarutkan dalam akuades sesuai dengan konsentrasi perlakuan yaitu 0%; 10%; 20%; 40%; dan 60% b/v). Biji Kacang Panjang (V. unguiculata) dan Padi (O. sativa) digunakan untuk melihat aktivitas daya hambat pertumbuhan bibit dari ekstrak Mikania. Uji allelopati dilakukan dalam skala laoratorium. Ke dalam cawan petri diletakkan kertas saring whatman, kemudian dibasahi dengan 6 ml larutan uji. Uji dilakukan tiga kali pengulangan, masing-masing 10 biji disiapkan untuk setiap pengulangan. Cawan Petri ditempatkan dalam ruang pertumbuhan di 220C. Panjang akar dan tunas ditentukan setelah 7 hari. Persentase penghambatan pertumbuhan akar dan panjang tunas dihitung dengan persamaan:
I(%) = (1-T/C)x100
T is the length of treatment (cm) and C is the length of control (cm).
Parameter Pengamatan Perkecambahan Paremeter perkecambahan yang diamati meliputi
persentase perkecambahan (%) serta panjang kecambah (cm) Kacang Hijau (Vigna radiata) dan Padi (Oriza sativa).. Pengambilan data dilakukan pada akhir pengamatan.

Results (English) 1:

3.3.5 Test Germination seeds of mung bean (Vigna radiata) and rice (Oriza sativa).Extract weighed and dissolved in akuades corresponds to a concentration of treatment i.e. 0%; 10%; 20%; 40%; and 60% of b/v). Chickpea seeds (v. unguiculata) and rice (o. sativa) are used to looking at the activities of seedling growth inhibitory power extracts of Mikania. Allelopati test is carried out in the scale of laoratorium. Into the petri dish was placed whatman filter paper, then moistened with 6 ml of the test solution. The test is performed three times each repetition, 10 seed prepared for any repetition. Petri dish was placed in space growth in 220C. The length of the roots and shoots was determined after 7 days. The percentage inhibition of the growth of root and shoot length calculated by the equation:I (percent) = (1-T/C) x 100T is the length of treatment (cm) and C is the length of control (cm).... The germination Germination Observation parameters observed includegermination percentage (percent) as well as the length of the sprouts (cm) mung bean (Vigna radiata) and rice (Oriza sativa.). Data retrieval is done at the end of the observation.