3) Penyimpanan dalam Minyak Mineral

3) Penyimpanan dalam Minyak Mineral
Salah satu cara sederhana untuk memelihara biakan bakteri, khamir dan jamur adalah dengan cara menyimpan dalam tabung agar miring dan menutup dengan minyak mineral atau parafin cair. Dasar teknik penyimpanan ini adalah mempertahankan viabilitas mikroba dengan mencegah pengeringan medium, sehingga waktu peremajaan dapat diperpanjang hingga beberapa tahun. Beberapa jenis jamur dapat bertahan hidup sampai 20 tahun. Daya tahan hidup mikroba lebih baik apabila biakan disimpan pada suhu kulkas (4o C). Mikroba yang akan dipelihara ditumbuhkan pada tabung berisi medium agar miring atau medium cair (broth) yang sesuai, kemudian permukaan biakan ditutup dengan minyak mineral steril setinggi 10-20 mm dari permukaan atas medium. Teknik ini sederhana, tetapi kurang praktis untuk ditransportasi. Di samping itu, keberadaan minyak mineral mengakibatkan peremajaan menjadi kotor.

Results (English) 1:

3) storage in Mineral oilOne of the simple ways to nurture the culture of bacteria, yeasts and fungi is by way of saving in a tube so that it tilts and closes with liquid paraffin or mineral oil. This storage technique is the basis of sustaining microbial viability by preventing the drying medium, so the time of rejuvenation can be extended up to several years. Some types of mold can survive up to 20 years. Durability of microbial life was better when kept at refrigerator temperature culture (4o C). Microbes that will be kept are grown on a medium containing agar slant tubes or liquid medium (broth), then the surface of the culture covered with sterile mineral oil as high as 10-20 mm from the upper surface of the medium. The technique is simple, but less practical to ditransportasi. In addition, the presence of mineral oil lead to rejuvenation of being dirty.

Results (English) 2:

3) Storage in Mineral Oil
One simple way to maintain a culture of bacteria, yeasts and fungi is by storing the tube for oblique and closing with mineral oil or liquid paraffin. Basic storage technique is to maintain the viability of microbes to prevent drying medium, thus rejuvenating time can be extended up to several years. Some types of fungi can survive up to 20 years. Microbial survival is better if the culture is stored at refrigerator temperature (4o C). Microbes that will be maintained grown in tubes containing agar slant or liquid medium (broth) is appropriate, then the culture surface covered with sterile mineral oil as high as 10-20 mm from the upper surface of the medium. This technique is simple, but it is not practical to transport. In addition, the presence of mineral oil resulted in the rejuvenation of being dirty.